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Alexey Bondar, Ph.D.

Pozice

Vědecký pracovník
Adresa
Zámek 136, 373 33 Nové Hrady
Tel.: +420389033821
bondar@nh.cas.cz

Dřívější výsledky

Education:

2014 Ph.D. degree in Biophysics at the Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic; thesis advisor: J. Lazar, Ph.D. Thesis title: “Studies of membrane protein structure and function using polarization microscopy”. Graduated with honors.

2006 Master’s degree in Biochemistry at the Belarusian State University in Minsk, Belarus; thesis advisor: M. Sholukh, Ph.D. Master’s thesis title: “Studies of the cytoprotective properties of prostaglandin groups A and E”. Graduated with honors.

2005 Bachelor’s degree in Biology at the Belarusian State University in Minsk, Belarus.


Employment History:

2017-present


2008-present



2015-2016

Postdoctoral fellow, Augusta University, USA – studies of G protein-coupled receptor signaling in native cells

Research fellow, Center for Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences, Czech Republic – studies of G protein signaling mechanisms in live cells

Research fellow (part time), Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Czech Republic – development of fluorescence microscopy techniques

2014 (6 months)


2013 (6 months)

Postdoctoral fellow, Tampere University of Technology, Finland – computational studies of G protein interactions with lipid membrane components

Visiting Research Fellow, Tampere University of Technology, Finland – computational studies of G protein interactions with G protein-coupled receptors

2008-2013

Research Assistant (part time), Faculty of Science, University of South Bohemia, Czech Republic – development of imaging techniques for studies of membrane proteins in live cells

2006-2007

Research Assistant (part time), Belarusian State University, Belarus – biochemical studies of antioxidant properties of synthetic prostaglandin analogs


Awards and Fellowships (selected):

2016 Keystone “Future of Science” scholarship to attend the conference “G Protein-Coupled Receptors: Structure, Signaling and Drug Discovery”, Keystone CO, USA.

2015 Award of the Czechoslovak Microscopy society for the best dissertation involving utilization of microscopy techniques

2014 Award of the University of South Bohemia for the dissertation with outstanding scientific contribution

2014 Keystone “Future of Science” scholarship to attend the conference “G protein-coupled receptors: structural dynamics and functional implications”, Snowbird UT, USA.

2012 FEBS Youth Travel Fund grant to attend the workshop “Dynamics of cell signal systems workshop”, Oslo, Norway

2010 FEBS Youth Travel Fund grant to attend the workshop “Spatial and temporal regulation of signaling”, Oslo, Norway

2009 FEBS Youth Travel Fund grant to attend the course “Practical course in computational biology”, Split, Croatia.

2009 EMBO travel fellowship to attend the course “Advanced optical microscopy”, UK.

2008 FEBS Youth Travel Fund grant to attend the course “Microspectroscopy: monitoring molecular interactions in living cells”, Wageningen, Holland.

2008 Student research grant of the Grant Agency of the University of South Bohemia



Publications (Total number of citations (WOS): 53; H-index 4):

  1. Sviridova E., Rezacova P., Bondar A., Veverka V., Novak P., Schenk G., Svergun D., Kuta Smatanova I., Bumba L. Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis Scientific Reports 7, 40408 (2017).

  2. Timr S., Brabec J., Bondar A., Ryba T., Zelezny M., Lazar J., Jungwirth P. Nonlinear Optical Properties of Fluorescent Dyes Allow for Accurate Determination of Their Molecular Orientations in Phospholipid Membranes Journal of Physical Chemistry B 110, 9706-9716 (2015).

  3. Han Z., Jin L., Chen F., Loturco J.J., Cohen L.B., Bondar A., Lazar J., Pieribone V.A. Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight. PLoS ONE 9(11): e113873 (2014).

  4. Bondar A., Lazar J. Dissociated GGTP and G protein subunits are the major activated form of heterotrimeric Gi/o proteins. Journal of Biological Chemistry 289, 1271-1281 (2014).

  5. Timr S., Bondar A., Cwiklik L., Stefl M., Hof M., Vazdar M., Lazar J. and Jungwirth P. Accurate Determination of the Orientational Distribution of a Fluorescent Molecule in a Phospholipid Membrane, Journal of Physical Chemistry B 118, 855-863 (2014)

  6. Lazar J., Bondar A., Timr S., Firestein S., Two-photon polarization microscopy reveals protein structure and function. Nature Methods 8, 684-690 (2011).

  7. Hubich A., Bondar A., Kastsiuk T., Kastsiuk U., Lakhvich F., Sholukh M., Hepatoprotective action of prostaglandin A2 analogs under CCl4-induced liver injury in vitro. Hepatology Research 37, 416-424 (2007).


Conference Presentations (selected):

2016 Bondar A., Brzakova A., Timr S., Lazar J. Precoupling of G proteins with GPCRs visualized by two-photon polarization microscopy // 60th Annual meeting of the Biophysical Society, Los Angeles CA, USA (Oral presentation)

2016 Bondar A., Lazar J. Studies of G proteins preassembly with GPCRs with two-photon polarization microscopy // G Protein-Coupled Receptors: Structure, Signaling and Drug Discovery, Keystone CO, USA (Oral presentation)

2015 Bondar A. Studies of protein structure and function using polarization microscopy // Annual meeting of Czechoslovak Microscopy Society, Lednice, Czech Republic (Invited talk)

2015 Bondar A., Timr S., Lazar J. G protein interactions with membrane components revealed by two-photon polarization microscopy // Focus on Microscopy, Gottingen, Germany (Poster)

2014 Bondar A., Lazar J. In vivo imaging and quantitation of interactions between Gi/o protein subunits and GPCRs by two-photon polarization microscopy // G protein-coupled receptors: structural dynamics and functional implications, Snowbird UT, USA (Oral presentation)

2013 Bondar A., Lazar J. G protein signaling studied by two-photon polarization microscopy // Seeing is believing – Imaging the processes of life, Heidelberg, Germany (Poster)


Tutoring of students:

2016 Molecular mechanism of G protein signaling. Annual Summer School in Molecular Biophysics and Systems Biology, Nove Hrady, Czech Republic (Project leader: 2 students)

2015 Regulation of G protein signaling by cell membrane components. Third Summer School in Molecular Biophysics and Systems Biology, Nove Hrady, Czech Republic (Project leader: 2 students)

2014 Molecular mechanisms of G protein signaling investigated by two-photon polarization microscopy. Second Summer School in Molecular Biophysics and Systems Biology, Nove Hrady, Czech Republic (Project leader: 2 students)

2012 Novel genetically-encoded sensors of cellular processes. Schola Ludus summer school program, Nove Hrady, Czech Republic. (Project leader: 2 students)

2011 Improved genetically-encoded calcium indicators for two-photon polarization microscopy. Schola Ludus summer school program, Nove Hrady, Czech Republic. (Project leader: 3 students)

2010 Studies of G protein interactions with receptors in live cells. Schola Ludus summer school program, Nove Hrady, Czech Republic. (Project leader: 2 students)

2009 A novel technique for monitoring G protein/GPCR activation in live cells, Schola Ludus summer school program, Nove Hrady, Czech Republic. (Project leader: 2 students)


Attended training courses:

2009 FEBS course “Practical course in computational biology”, Split, Croatia.

2009 EMBO practical course “Advanced optical microscopy”, Plymouth, UK.

2008 FEBS course “Microspectroscopy: monitoring molecular interactions in living cells”, Wageningen, Holland.


Scientific Interests:

- Mechanisms of GPCR/G protein signal transduction

- Imaging and computational techniques for studies of membrane proteins


Member of:

American Society for Neuroscience

American Society for Biochemistry and Molecular Biology

Biophysical society

Czech Society for Biochemistry and Molecular Biology (FEBS constituent society)

Czechoslovak Microscopy Society (EMS constituent society)


Languages Spoken:

Fluent: Russian (native), English (advanced); other: Belarusian, Czech, German

Ústavní publikace v impaktovaných časopisech