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Mgr. Katerina Shamayeva, PhD.

Pozice

Postdoktorand
Adresa
Zamek 136, 37333 Nove Hrady
shamayeva@nh.cas.cz

Dřívější výsledky

EDUCATION

From 18.12.2015: PhD in Biophysics (Thesis was written in English)
Thesis: Involvement of the endonuclease domain of the EcoR124I restriction-modification complex in interdomain communication (http://theses.cz/id/9zim7o?info=1;isshlret=SHAMAYEVA)
2008-2015: Faculty of Science of University of South Bohemia in Ceske Budejovice, PhD student, program Biophysics
2003-2008: Department of Zoology, Belarusian State University, Minsk, Belarus. Diploma degree in Biology and Chemistry

EMPLOYMENT
2016-present: Center for Nanobiology and Structural Biology, Institute of Microbiology, Academy of the Science of Czech Republic, Department of Structure and Function of Proteins, postdoc
2011-2015: employee at Institute of Nanobiology and Structural Biology GCRC ASCR, Department of Structure and Function of Proteins
2010-2014: employee at Faculty of Science of University of South Bohemia in Ceske Budejovice, Department Chemistry and Biochemistry

TEACHING EXPERIENCE (ONLY IN ENGLISH)
2011, July: International Summer School SCHOLA LUDUS, project leader in project “Bacterial transformation efficiency and site-direct mutagenesis with following expression of mutant”.2012-present: Protein Chemistry course – laboratory exercises “Fluorescence”, “Restriction- modification systems”, “Proteolysis”
2013, 8th July-27th July: First Summer School in Molecular Biophysics and Systems Biology, project leader in project “DNA-binding proteins. Purification and Properties”.
2014, 7th July-27th July: Second Summer School in Molecular Biophysics and Systems Biology, project leader in project “Expression, Purification, Restriction and ATPase activities in mutated subunit HsdR from EcoR124I restriction-modification complex”.
2013-2015: contact person in First, Second and Third Summer Schools in Molecular Biophysics and Systems Biology

WORK EXPERIENCE
Local practice: 30th January-10th February, 2012 in Dr. Marie Weiserova’s lab at the Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
International practice: 1th July-31th August 2012 in Dr. Jost Ludwig’s lab at the IZMB at Bonn University, Germany

MEMBERSHIP
2014-present: Member of the Czech Society for Biochemistry and Molecular Biology, the FEBS Constituent Society

SKILLS
Experimental Skills
DNA/RNA isolation, DNA electrophoresis, PCR
Basic techniques in molecular biology (cloning, restriction analysis, site-directed mutagenesis, competent cells preparation and transformation)
Expression of recombinant proteins in E. coli Recombinant proteins purification (affinity chromatography, ion Exchange chromatography, gel filtration)
Analysis of proteins (SDS-PAGE, proteolysis, in vitro assays, data analysis) In vivo assays (working with lambda phage: phage preparation, transformation, titration ect.)
Crystallization basic techniques (protein preparation, vapor diffusion methods, pre crystallization test, cryoprotections, data collections)
Experience with yeast

Computer Skills
MS Windows, Yasara, Gene Tools, Image J, Sigma Plot

Language Skills
Russian
English
Czech
Polish

Other Skills
From 2008: Driving License B

LIST OF PUBLICATIONS
Sinha, D*., Shamayeva, K*., Ramasubramani, V., Reha, D., Bialevich, V., Khabiri, M., Milbar, N., Guzanova, A., Weiserova, M., Csefalvay, E., Carey, J., Ettrich R. (2014) Interdomain communication in the endonuclease/motor subunit of Type I restriction-modification enzyme EcoR124I Journal of Mol Model. 20, 2334. *contributed equally, IF=1.867
Eva Csefalvay, Mikalai Lapkouski, Alena Guzanova, Ladislav Csefalvay, Tatsiana Baikova, Igor Shevelev, Vitali Bialevich, Katsiaryna Shamayeva, Pavel Janscak, Ivana Kuta Smatanova, Santosh Panjikar, Jannette Carey, Marie Weiserova, Rüdiger Ettrich (2015) Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme PLoS ONE 10 (6): e0128700, IF=3.534
Vitali Bialevich, Dhiraj Sinha, Katsiaryna Shamayeva, Alena Guzanova, David Řeha, Eva Csefalvay, Jannette Carey, Marie Weiserova, Rüdiger H Ettrich (2017) The helical domain of the EcoR124I motor subunit participates in ATPase activity and dsDNA translocation PeerJ 5: e2887, IF=2.183

INTERESTS
Sport
Travelling
Reading
Bicycling